Bioinformatics analysis for next generation sequencing is divided into different types of analyses, depending on the application. There are different approaches, whether a de novo assembly needs to be performed or a reference sequence is provided by the customer. In the latter case of re-sequencing, the most common application is exome analysis. Specially enriched target regions address the exons of a genome, only. A detailed discussion and collaboration of the customer's needs is essential and is part of any next generation sequencing project performed at ATLAS Biolabs GmbH.